Mobile phases in HPLC
Mobile phase is the most important parameter in reversed-phase HPLC. Type of mobile phase
used may have a big effect on the retention. It can promote or suppress an ionization of the
analyte molecules, and it also can shield an accessible residual silanol or any other active
adsorption centers on the adsorbent surface.
Proper selection of the mobile phase is the second most important step in the development of the
separation method (the first one is the selection of the adsorbent type). The main requirement for
the mobile phase is that it has to dissolve the analytes up to the concentration suitable for the
detection. Variation of the eluent composition provides the great flexibility of HPLC separations.
Related Conference of Mobile phases in HPLC
March 13-14, 2025
2nd International Conference on Drug Discovery and Development
Prague, Czech Republic
April 07-08, 2025
17th International Conference & Expo on Chromatography Techniques
Vancouver, Canada
June 25-26, 2025
15th World Congress on Analytical & Bioanalytical Techniques
Aix-en-Provence, France
September 25-26, 2025
25th International Conference on Medicinal Chemistry and Drug Design
Zurich, Switzerland
Mobile phases in HPLC Conference Speakers
Recommended Sessions
- Â Proteomics from Discovery to Function and its applications
- Mass Spectrometry in Proteome Research
- Analytical Chemistry
- Application Of Chromatography
- Application Of Mass Spectrometry
- Chromatography and High Performance Liquid Chromatography (HPLC)
- Clinical Chemistry And Laboratory Techniques
- Components Of Mass Spectrometery
- Crystallography
- Fundamentals Of Mass Spectrometry
- Ionization Techniques
- Maintenanc,Troubleshooting, Data Analysis and Experimentation in mass spectrometry
- Mass Spectrometry Configurations and Sample preparation Techniques
- Mass Spectrometry Imaging
- Mass Spectrometry Of Polymers
- Mobile phases in HPLC
- New Approaches In Mass Spetrometry
- Principle Of Mass Spectrometrty
- Principles of chromatography
- Recent Advances In Mass Spetrometry
- Resolution in HPLC
- RT and RRT in HPLC
- Spectrometry
- Spectroscopy Tools In Nanotechnology
- Tandem Mass Spectroscopy
Related Journals
Are you interested in
- 3D Printing for Drug Delivery - DRUG DISCOVERY 2025 (Czech Republic)
- Advanced Medicinal Chemistry - Chemistry Meeting-2025 (France)
- Advanced Organic & Inorganic Chemistry - Chemistry Meeting-2025 (France)
- Advancements in Mass Spectrometry - Analytical Techniques 2025 (France)
- Advancements in Point-of-Care Testing - Clinical Chemistry 2025 (Canada)
- Advances in Biomarker Discovery and Application - Clinical Chemistry 2025 (Canada)
- Advances in Biopolymer Science - biopolymerscongress-2025 (Spain)
- Advances in Chromatography Instrumentations - Chromatography Techniques-2025 (France)
- Advances in Chromatography-HPLC Instrumentations - Advanced Chromatography 2025 (Canada)
- Advances in Injectable Drugs - DRUG DISCOVERY 2025 (Czech Republic)
- Advances in Separation Techniques - Analytical Techniques 2025 (France)
- Agricultural Chemistry - Chemistry Meeting-2025 (France)
- AI in Drug Delivery - DRUG DISCOVERY 2025 (Czech Republic)
- Analytical & Bio-analytical Applications of Chromatography - Chromatography Techniques-2025 (France)
- Analytical & Bio-analytical Applications of Chromatography - Advanced Chromatography 2025 (Canada)
- Analytical and Bio analytical Chemistry - World Chemistry 2025 (Canada)
- Analytical Chemistry - Chemistry Meeting-2025 (France)
- Analytical Chemistry and Methodology - Analytical Techniques 2025 (France)
- Applications of mass spectrometry - Analytical Techniques 2025 (France)
- Applications of Mass Spectrometry in Clinical Chemistry - Clinical Chemistry 2025 (Canada)
- Applied Chemistry - Chemistry Meeting-2025 (France)
- Bio-Based Polymers for Packaging - biopolymerscongress-2025 (Spain)
- Bioanalytical Methodology - Analytical Techniques 2025 (France)
- Biochemical Applications of Chromatography - Chromatography Techniques-2025 (France)
- Biochemical Applications of Chromatography-HPLC - Advanced Chromatography 2025 (Canada)
- Biochemistry - Chemistry Meeting-2025 (France)
- Biochemistry - Analytical Techniques 2025 (France)
- Biodegradable Polymers - biopolymerscongress-2025 (Spain)
- Biopolymer Synthesis Techniques - biopolymerscongress-2025 (Spain)
- Biopolymers and Biomaterials - Analytical Techniques 2025 (France)
- Biopolymers in Drug Delivery Systems - biopolymerscongress-2025 (Spain)
- Biotechnology - World Chemistry 2025 (Canada)
- Brain and Nervous System Drug Delivery - DRUG DISCOVERY 2025 (Czech Republic)
- Breathing and Nasal Drug Delivery - DRUG DISCOVERY 2025 (Czech Republic)
- Challenges in Drug Formulation - DRUG DISCOVERY 2025 (Czech Republic)
- Chemical Engineering - Chemistry Meeting-2025 (France)
- Cheminformatics & Big Data in Chemistry - Chemistry Meeting-2025 (France)
- Chemistry in Clinical Research - Chemistry Meeting-2025 (France)
- Chip Based Chromatography Separations - Chromatography Techniques-2025 (France)
- Chip Based Chromatography Separations - Advanced Chromatography 2025 (Canada)
- Chromatography - Analytical Techniques 2025 (France)
- Chromatography & Mass Spectrometry - Advanced Chromatography 2025 (Canada)
- Chromatography as Separation Techniques - Chromatography Techniques-2025 (France)
- Chromatography in Bio-Medical Research - Chromatography Techniques-2025 (France)
- Chromatography in Food Science Technology - Chromatography Techniques-2025 (France)
- Chromatography in Food Science Technology - Advanced Chromatography 2025 (Canada)
- Chromatography in Pharmacy & Pharmaceutical - Chromatography Techniques-2025 (France)
- Chromatography in Pharmacy & Pharmaceutical - Advanced Chromatography 2025 (Canada)
- Chromatography-HPLC as Separation Techniques - Advanced Chromatography 2025 (Canada)
- Chromatography-HPLC in Bio-Medical Research - Advanced Chromatography 2025 (Canada)
- Chromatography-Mass Spectroscopy Analysis - Chromatography Techniques-2025 (France)
- Clinical and Diagnostic Bioanalysis - Analytical Techniques 2025 (France)
- Clinical Chemistry in Oncology - Clinical Chemistry 2025 (Canada)
- Clinical Chemistry in Precision Medicine - Clinical Chemistry 2025 (Canada)
- Clinical Diagnostics Equipment and Kits - Analytical Techniques 2025 (France)
- Computational Chemistry - Chemistry Meeting-2025 (France)
- Computational Chemistry and Chemical Engineering - World Chemistry 2025 (Canada)
- Crystal engineering - Chemistry Meeting-2025 (France)
- Data Management and Informatics in Clinical Chemistry - Clinical Chemistry 2025 (Canada)
- Delivering Peptide and Protein Drugs - DRUG DISCOVERY 2025 (Czech Republic)
- Development of Biologic Drugs - DRUG DISCOVERY 2025 (Czech Republic)
- Developments in Genetic and Genomic Testing - Clinical Chemistry 2025 (Canada)
- Drug Delivery in Cancer Treatment - DRUG DISCOVERY 2025 (Czech Republic)
- Drug Delivery in Regenerative Medicine - DRUG DISCOVERY 2025 (Czech Republic)
- Electro Chemistry - World Chemistry 2025 (Canada)
- Electrochemistry and Energy Storage - Chemistry Meeting-2025 (France)
- Electrophoresis - Analytical Techniques 2025 (France)
- Emerging Trends in Analytical Techniques - Clinical Chemistry 2025 (Canada)
- Environmental & Green Chemistry - World Chemistry 2025 (Canada)
- Environmental Analytical Chemistry - Analytical Techniques 2025 (France)
- Environmental Chemistry and Geochemistry - Chemistry Meeting-2025 (France)
- Environmental Impact of Biomaterials - biopolymerscongress-2025 (Spain)
- Ethics and Regulations in Clinical Chemistry - Clinical Chemistry 2025 (Canada)
- Eye Drug Delivery Techniques - DRUG DISCOVERY 2025 (Czech Republic)
- Food and Flavor Chemistry - Chemistry Meeting-2025 (France)
- Food Chemistry & Agricultural Chemistry - World Chemistry 2025 (Canada)
- Food Industry Applications of Biopolymers - biopolymerscongress-2025 (Spain)
- Future Directions in Clinical Chemistry - Clinical Chemistry 2025 (Canada)
- Future Trends in Biopolymers - biopolymerscongress-2025 (Spain)
- Green Chemistry - Chemistry Meeting-2025 (France)
- Green Chemistry in Polymer Development - biopolymerscongress-2025 (Spain)
- High Efficiency & High Resolution Techniques - Chromatography Techniques-2025 (France)
- High Efficiency & High Resolution Techniques - Advanced Chromatography 2025 (Canada)
- HPLC Fingerprinting in Bioinformatics & Computational Biology - Chromatography Techniques-2025 (France)
- HPLC Fingerprinting in Bioinformatics & Computational Biology - Advanced Chromatography 2025 (Canada)
- Hyphenated Chromatography Methods - Chromatography Techniques-2025 (France)
- Hyphenated Chromatography Methods - Advanced Chromatography 2025 (Canada)
- Immunotherapy and Vaccine Delivery - DRUG DISCOVERY 2025 (Czech Republic)
- Improvements in Oral Drugs - DRUG DISCOVERY 2025 (Czech Republic)
- Improving Drug Absorption - DRUG DISCOVERY 2025 (Czech Republic)
- Industrial Applications of Biomaterials - biopolymerscongress-2025 (Spain)
- Industrial Chemistry - Chemistry Meeting-2025 (France)
- Innovation in Food Analysis & Testing - Analytical Techniques 2025 (France)
- Innovations in Diagnostic Technologies - Clinical Chemistry 2025 (Canada)
- Innovations in Polymer Chemistry - biopolymerscongress-2025 (Spain)
- Integration of Artificial Intelligence in Diagnostics - Clinical Chemistry 2025 (Canada)
- Major Chromatographic Techniques - Chromatography Techniques-2025 (France)
- Major Chromatographic Techniques - Advanced Chromatography 2025 (Canada)
- Marine Chemistry and Geochemistry - World Chemistry 2025 (Canada)
- Marine-Derived Biopolymers - biopolymerscongress-2025 (Spain)
- Market Growth of Chromatography - Chromatography Techniques-2025 (France)
- Market Growth of Chromatography-HPLC - Advanced Chromatography 2025 (Canada)
- Mass Spectroscopy & Chromatography - World Chemistry 2025 (Canada)
- Materials Chemistry - Chemistry Meeting-2025 (France)
- Medical Applications of Biomaterials - biopolymerscongress-2025 (Spain)
- Medicinal Chemistry - World Chemistry 2025 (Canada)
- Method Development & Validation - Chromatography Techniques-2025 (France)
- Method Development & Validation - Advanced Chromatography 2025 (Canada)
- Microscopy - Analytical Techniques 2025 (France)
- Nanoscience & Material Chemistry - World Chemistry 2025 (Canada)
- Nanotechnology in Biomaterials - biopolymerscongress-2025 (Spain)
- Natural Product Chemistry - Chemistry Meeting-2025 (France)
- Natural Product Chemistry - Chemistry Meeting-2025 (France)
- New Drug Delivery Methods - DRUG DISCOVERY 2025 (Czech Republic)
- New Frontiers in Clinical Chemistry Research - Clinical Chemistry 2025 (Canada)
- New Instrumentation and Equipment - Analytical Techniques 2025 (France)
- NMR and Analysis of small Organic Molecules - Analytical Techniques 2025 (France)
- Novel Approaches to analytical and Bioanalytical method - Analytical Techniques 2025 (France)
- Nuclear Chemistry - World Chemistry 2025 (Canada)
- Oil and Petroleum Chemistry - World Chemistry 2025 (Canada)
- Pharmaceutical Analytics - Analytical Techniques 2025 (France)
- Pharmaceutical Chemistry - Chemistry Meeting-2025 (France)
- Physical Chemistry - Chemistry Meeting-2025 (France)
- Physical Chemistry & Theoretical Chemistry - World Chemistry 2025 (Canada)
- Polymer Chemistry - Chemistry Meeting-2025 (France)
- Polymer Chemistry - World Chemistry 2025 (Canada)
- Polymer Composites and Applications - biopolymerscongress-2025 (Spain)
- Polymer Recycling and Sustainability - biopolymerscongress-2025 (Spain)
- Polymer Science and Material Science - Analytical Techniques 2025 (France)
- Proteomics and its applications - Analytical Techniques 2025 (France)
- Quality Control and Assurance in Clinical Laboratories - Clinical Chemistry 2025 (Canada)
- Real-World Examples of Drug Delivery - DRUG DISCOVERY 2025 (Czech Republic)
- Recent Advances in Chromatography - Chromatography Techniques-2025 (France)
- Recent Advances in Chromatography-HPLC - Advanced Chromatography 2025 (Canada)
- Regulatory and Market Challenges - DRUG DISCOVERY 2025 (Czech Republic)
- Regulatory Issues and Biosafety Challenges in Bio analysis - Analytical Techniques 2025 (France)
- Renewable Resources for Biomaterials - biopolymerscongress-2025 (Spain)
- Skin-Based Drug Delivery - DRUG DISCOVERY 2025 (Czech Republic)
- Smart Biomaterials for Healthcare - biopolymerscongress-2025 (Spain)
- Sustainable and Ethical Drug Development - DRUG DISCOVERY 2025 (Czech Republic)
- Sustainable Biomaterials - biopolymerscongress-2025 (Spain)
- Targeted and Personalized Drug Delivery - DRUG DISCOVERY 2025 (Czech Republic)
- The Role of Clinical Chemistry in Infectious Disease Diagnostics - Clinical Chemistry 2025 (Canada)
- Theoretical Chemistry - Chemistry Meeting-2025 (France)
- Tissue Engineering and Biomaterials - biopolymerscongress-2025 (Spain)
- UV Spectroscopy - Analytical Techniques 2025 (France)
- Water Chemsitry - World Chemistry 2025 (Canada)