Ionization Techniques
There are many types of ionization techniques are used in mass spectrometry methods. The
classic methods that most chemists are familiar with are electron impact (EI) and Fast Atom
Bombardment (FAB). These techniques are not used much with modern mass spectrometry
except EI for environmental work using GC-MS. Electrospray ionization (ESI) - ESI is the
ionization technique that has become the most popular ionization technique. The electrospray is
created by putting a high voltage on a flow of liquid at atmospheric pressure, sometimes this is
assisted by a concurrent flow of gas. Atmospheric Pressure Chemical Ionization (APCI) - APCI
is a method that is typically done using a similar source as ESI, but instead of putting a voltage
on the Electrospray Tandem Mass Spectrometry Newborn Screening itself, the voltage is placed
on a needle that creates a corona discharge at atmospheric pressures. Matrix Assisted
Laser Electrophoresis is a technique of ionization in which the sample is bombarded with a laser.
The sample is typically mixed with a matrix that absorbs the radiation biophysics and transfer a
proton to the sample. Gas-Phase Ionization.
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