Chromatography and High Performance Liquid Chromatography (HPLC)

Liquid chromatography-mass spectrometry analysis of small molecules from biofluids requires
sensitive and robust assays. Because of the very complex nature of many biological samples,
efficient sample preparation protocols to remove unwanted components and to selectively extract
the compounds of interest are an essential part of almost every bioanalytical workflow. High-
performance liquid chromatography (HPLC) is a separation technique that can be used for the
analysis of organic molecules and ions. HPLC is based on mechanisms of adsorption, partition
and ion exchange, depending on the type of stationary phase used. HPLC involves a solid
stationary phase, normally packed inside a stainless-steel column, and a liquid mobile phase.
Separation of the components of a solution results from the difference in the relative distribution
ratios of the solutes between the two phases. HPLC can be used to assess the purity and/or
determine the content of many pharmaceutical bioprocessing substances. It can also be used to
determine enantiomeric composition, using suitably modified mobile phases or chiral stationary
phases. Individual separation mechanisms of adsorption, partition and ion exchange rarely occur
in isolation since several principles act to a certain degree simultaneously in a very
environmental monitoring, hydrophilic molecules will tend to associate with each other (like
water drops on an oily surface). The hydrophilic molecules in the mobile phase will tend to
adsorb to the surface on the inside and outside of a particle if that surface is also hydrophilic.
Increasing the polarity of the mobile phase will subsequently decrease the adsorption and
ultimately cause the sample molecules to exit the column. This mechanism is called Normal
Phase Analytical Chromatography. It is a very powerful technique that often requires non-polar
solvents. Due to safety and environmental concerns this mode is used mostly as an analytical
technique and not for process applications.

    Related Conference of Chromatography and High Performance Liquid Chromatography (HPLC)

    November 27-28, 2024

    14th World Congress on Analytical & Bioanalytical Techniques

    Amsterdam, Netherlands
    November 28-29, 2024

    20th European Organic Chemistry Congress

    Paris, France
    December 12-13, 2024

    23rd World Congress on Nutrition and Food Chemistry

    Rome, Italy
    December 18-19, 2024

    4th International Conference on Petrochemistry and Natural Gas

    Amsterdam, Netherlands
    January 27-28, 2025

    25th World Congress on Medicinal Chemistry and Drug Design

    Bangkok, Thailand
    March 13-14, 2025

    2nd International Conference on Drug Discovery and Development

    Prague, Czech Republic
    April 07-08, 2025

    17th International Conference & Expo on Chromatography Techniques

    Vancouver, Canada
    April 07-08, 2025

    15th World Congress on Chemistry

    Vancouver, Canada
    May 14-15, 2025

    17th International Conference on Clinical Chemistry

    Toronto, Canada
    May 28-29, 2025

    15th International Conference on Chemistry Meeting

    Paris, France
    June 26-27, 2025

    14th World Congress on Chromatography

    Paris, France

    Chromatography and High Performance Liquid Chromatography (HPLC) Conference Speakers

      Recommended Sessions

      Related Journals

      Are you interested in