Maintenanc,Troubleshooting, Data Analysis and Experimentation in mass spectrometry

Mass spectrometry experiment (MS) is a high-throughput experimental method that characterizes
molecules by their mass-to-charge ratio. The MS is composed of sample preparation, molecular
ionization, detection, and instrumentation analysis processes. MS is beneficial in that it is generally fast,
requires a small amount of sample, and provides high accuracy measurements. For these reasons, MS
alone or combined with other structural proteomics techniques is widely used for various molecular
biology analysis purposes. Examples of the analysis include post-translations modifications in proteins,
identification of vibrational components in proteins, and analysis of protein conformation and dynamics.
We will focus on MS-coupled methods that provide information about conformation and dynamics of the
protein being studied. For a comprehensive review on MS procedures and for a review on various types of
MS-coupled methods. The performance of a mass spectrometer will be severely impaired by the lack of a
good vacuum in the ion transfer region of the mass Analyzer.  As the vacuum deteriorates it will become
insufficient to maintain biomedical instrumentation in the operating mode. If the foreline pump is not
maintained, the oil may become so contaminated that the optimum pumping is no longer
possible.  Initially, gas transport and metabolism ballasting may clean the oil.  If the oil has become
discoloured then it should be changed according to the pump manufacturers’ maintenance manual.  When
rotary pumps are used to pump away conflict resolution, the solvent can  become dissolved in the oil
causing an increase in backing line pressure.  Gas ballasting is a means of purging the oil to remove
dissolved contaminants

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