GAS CHROMATOGRAPHY AND HPLC


In gas chromatography gas is the mobile phase. The two main division of gas chromatography is GAS –SOLID CHROMATOGARAPHY (GSC) and GAS –LIQUID CHROMATOGRAPHY (GLC). usually used for thermally stable compounds. mobile phase is called carrier gas, they are inert in nature generally used gases are helium, nitrogen, argon etc.  HPLC or high performance liquid chromatography is improved form of liquid chromatography. High pressure up to 400 atm is used. this is much faster method. both small particles and large surface area particles can easily be separated. A combination of high pressure and absorbent of small size results in high resolution power and less analysis time of sample.


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