Mass Spectrometry and Chromatography

CLINICAL APPLICATION OF CHROMATOGRAPHY

One of the major challenges in the medicine today is developing new therapies that improve human health. To help address these challenges the utilization of analytical technologies has been employed; to perform more experiments in a shorter time with increased data quality. In the last years various analytical strategies have been established to enhance separation speed and efficiency in liquid chromatography applications. Liquid chromatography is an important tool for monitoring drugs and their metabolites. Additionally, liquid chromatography has played an important role in pharmacokinetics and metabolism studies at these drug development stages. Current trends in fast liquid chromatographic separations involve technologies, fused-core columns, high-temperature liquid chromatography (HTLC) and ultra-high performance liquid chromatography (UHPLC). The high specificity in combination with high sensitivity makes it an attractive method to traditional methodology used for daily applications.