ADVANCEMENT OF CHROMATOGRAPHIC INSTRUMENTS


HPLC is a popular method of analysis for natural products because of its high accuracy and is not differed by the stability or the volatility of the compounds. HPLC combined with diode array detector (HPLC-DAD), mass spectrometer (HPLC-MS) has been utilized for the qualitative and quantitative determination of various types of Phyto-constituents like alkaloids, glycosides, tannins, tri-terpenes, etc. HPLC methods are used for analyzing of drug in biological fluids and pharmaceutical dosage forms. HPLC determination with spectroscopic detection is useful for daily quality control of drugs in pharmaceutical dosage forms and studies.



A chromatographic detector can create both identity and concentration elution of components in the mobile phase stream. Various range of detectors are available to meet different sample conditions. some detectors are UV-VIS, Photo diode array, fluorescence, and mass spectroscopic detectors. Some bulk Property detectors include refractive index electrochemical and light scattering detectors.


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