Mass Spectrometry and Chromatography

ANALYSIS OF BIOMOLECULES WITH MASS SPECTROMETRY

Mass spectrometry is becoming vital field for analyzing biomolecules. Earlier electrophoresis and ultracentrifugation were used for similar information. But results were not as per demand because they are dependent on several ither factors also. Thus, only option left was calculation which was time consuming. Development of spectrometric technique not only saves times but help to analyze the structure of complex compounds.

Analysis of glycans/ lipids

Glycans are formed from several monosaccharides through glycosidic linkage which makes it complex to analyze. mass spectrometry is emerging technique for glycemic and glycobiology.

Lipids are made up of different class molecules. Mass spectrometric techniques help in analyzing such complex compounds. Most recent techniques are ESI (electrospray ionization) and triple quadrupole analyzers. Using MS, it is easy to determine structure molecular weight and branching of compounds.