APPLICATION OF CHROMATOGRAHPHY


Chromatographic technique will separate ionic species, inorganic or organic, and molecular species varying in size from the lightest and smallest, helium and hydrogen, to particulate matter such as single cells. It will separate several hundreds of components of unknown identity and concentrations, leaving the components unchanged. Amounts in the parts per billion range can be analyzed with some detectors. The solutes can range from polar to nonpolar. 



Separations are fast varying from analysis times of a few minutes to several hours. The earlier world would have considered a time of several hours to separate multicomponent mixtures to be miraculously fast. Now several hours is reduced and there is much emphasis on increasing speed.


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